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KMID : 0381920090390020185
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Korean Journal of Microscopy
2009 Volume.39 No. 2 p.185 ~ p.189
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Three Dimensional Reconstruction of Cellular Structure in Drosophila Retina Using High Voltage Electron Microscopy
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Moon Ji-Young
Lee Kyung-Eun
Han Sung-Sik
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Abstract
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Studies about the structure of Drosophila melanogaster retinal cell using electron microscopy have been carried out in details since 1960s. However, these results can have limitations in functional research because of two-dimensional structure. In this study, the adult retina of Drosophila melanogaster was investigated by employing high pressure freezing method, serial sections, high voltage electron microscopy, and 3-dimensional reconstruction method. From there results, mitochondria, microtubules, and nuclei were reconstructed as 3-dimensional structure using IMOD program. The 3D structure of these organelles showed that mitochondira mainly located in distal region near lens, and microtubule mainly located in distal and basal region. The 3D reconstruction of these organelles can be used for a critical evaluation in the dynamic change of cellular organelles caused by functional abnormality like retinal degeneration.
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KEYWORD
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HVEM, 3D structure, Retina, Drosophila melanogaster
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